All data are available within the Article and Supplementary Files. The scRNAseq datasets have been deposited in the NCBI Gene Expression Omnibus database ( ) with the accession code number "type":"entrez-geo","attrs":"text":"GSE166981","term_id":"166981"GSE166981. The scRNAseq analysis was performed with standard pipelines in Seurat R packages, as described in the Methods. Scripts will be made available upon request. The data and detailed protocols in this manuscript are available from the authors upon request. Source data are provided with this paper.
Glenda Model Sets 59 To 67
J.E.d.L. initiated the project, conducted the BMP experiments, contributed and analysed the mouse work and performed lateral plate electroporations. C.B. conducted lateral plate grafting experiments, lateral plate electroporations, in situ hybridisation and immunohistochemistry to experimental chicken and mouse limbs. M.-A.B. contributed to somite grafting experiments, performed cell preparation for scRNAseq and analysed the mouse work. E.H. performed confocal imaging of chicken embryos and conducted the bioinformatic analysis of the scRNAseq datasets. G.C. conducted the mouse work and immunohistochemistry for myogenic markers. L.Y. performed G.F.P. chicken (somite and neural tube) and quail (somite) grafting experiments. M.-C.D. performed lateral plate electroporation in chicken embryos. L.B. and S.M. contributed to the design of the scRNAseq analysis methods. R.S. provided the Scx:Cre embryos. S.N. and C.F.-T. performed qPCR on fibroblasts. TJ performed cell preparation for scRNAseq. J.E.d.L., E.H., G.C., L.Y., C.R., C.F.-T., S.T. and F.R. discussed the project and contributed to writing the manuscript. D.D. supervised the study, analysed the results, pictured the data and organised the figures, wrote the manuscript and acquired funding.
He worked as a roofer until 10 years ago when he suffered a workplace accident that forced him to retire, his sister said. Since then, he spent his time building military vehicle models and hanging out at Fox Airfield in Antelope Valley to watch planes take off and land. He also loved Slipknot, the heavy metal band. 2ff7e9595c
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